Chloroplast DNA topoisomerase I from pea leaves
نویسندگان
چکیده
منابع مشابه
Lipid Composition of Pea and Bean Leaves during Chloroplast Development.
The changes in composition of the complex lipids were followed during the greening of dark-grown pea (Pisum sativum) and bean (Phaseolus vulgaris) seedlings. No significant changes in glycerolipid concentrations in the leaves were observed during the early stages of greening (0-8 hour for peas and 0-12 hour for beans). On further greening, there was an increase in the proportion of galactolipid...
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DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg2+, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.
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The mitochondrial DNA from pea leaves exists in a circular conformation. 25% of the circular molecules exist as supercoils, and 10% of the molecules are dimers. The molecular weight of mitochondrial DNA is about 66 to 70 x 10(6) by electron microscopy, and 74 x 10(6) from its renaturation kinetics. No evidence for inter- and intramolecular heterogeneity is found.
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Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
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Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1991
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0002e9